HCG

Product Overview

Human Chorionic Gonadotropin (HCG) is a glycoprotein hormone composed of two non-covalently linked subunits (alpha and beta) that plays a critical role in reproductive biology research. Naturally produced by trophoblastic cells, HCG has become one of the most widely studied hormones in endocrinology, fertility research, and metabolic investigation. Its ability to bind luteinizing hormone (LH) receptors makes it an invaluable tool for researchers studying gonadal function, steroidogenesis, and hypothalamic-pituitary-gonadal axis regulation.

NXTSTATE supplies HCG in research-grade lyophilized form, verified by independent third-party analysis for potency, purity, and biological activity. Each vial ships with a Certificate of Analysis documenting batch-specific quality metrics to ensure reproducible experimental results.

Research Background

HCG exerts its biological effects by binding to LH/CG receptors expressed on gonadal cells, activating adenylate cyclase and increasing intracellular cyclic AMP (cAMP) levels. This cascade stimulates steroid hormone biosynthesis, particularly testosterone in Leydig cells and progesterone in luteal cells. Researchers have extensively utilized HCG as a tool for studying gonadotropin receptor signaling, Leydig cell function, and ovarian physiology in both in vitro and in vivo experimental models.

Beyond reproductive biology, HCG research has expanded into areas including angiogenesis, immune modulation, and metabolic regulation. The hormone isoform profile and glycosylation patterns have been subjects of detailed investigation, as these structural features significantly influence biological half-life, receptor binding affinity, and downstream signaling potency. HCG remains a cornerstone compound in endocrine research laboratories worldwide.

Product Specifications

Molecular Weight: Approximately 36,700 Da (heterodimeric glycoprotein) | Purity: Minimum 97% by SDS-PAGE and HPLC | Biological Activity: Verified by in vitro bioassay | Form: Lyophilized powder | Storage: Store at -20 degrees Celsius for long-term stability. Reconstituted solutions should be aliquoted and stored at -20 degrees Celsius. Avoid repeated freeze-thaw cycles.

Quality Assurance

NXTSTATE ensures every batch of HCG meets stringent quality standards through comprehensive third-party analytical testing. Quality control protocols include SDS-PAGE for subunit integrity verification, reverse-phase HPLC for purity assessment, and biological activity assays to confirm functional potency. Endotoxin testing is performed to ensure suitability for sensitive research applications. Each shipment includes a detailed Certificate of Analysis reflecting batch-specific results across all quality parameters. Our supply chain maintains cold-chain integrity from manufacture through delivery.

Reconstitution and Handling

HCG is supplied as a lyophilized powder and should be reconstituted with sterile bacteriostatic water or appropriate buffered saline solution. Gentle swirling is recommended during reconstitution to preserve protein integrity and avoid denaturation. Reconstituted solutions should be aliquoted into single-use volumes and stored at -20 degrees Celsius. Researchers should avoid vigorous vortexing or exposure to elevated temperatures. All handling should follow standard laboratory biosafety protocols including appropriate personal protective equipment.

Compliance and Disclaimers

This product is sold strictly for research use only. It is not intended for human consumption, therapeutic use, or diagnostic purposes. NXTSTATE products are manufactured and distributed in compliance with all applicable regulations governing research chemicals and biological materials. Purchasers assume full responsibility for ensuring compliance with institutional review board requirements and applicable local, state, and federal regulations. NXTSTATE does not make any claims regarding therapeutic efficacy or safety for human or veterinary use.